Enzymes used for food supplements
These are enzymes that are used in direct ingestion applications. They are often components of tablets marketed as digestive aids. Some are used as single ingredient components, such as Lactase, Bromelain, or Papain
PROTEASES
Papain:
A natural protease from papaya
Papain is a sulfhydryl protease extracted from the water-soluble portion of the latex of the fruit of Carica Papaya. It is a versatile protease that will rapidly hydrolyze a variety of proteins over a wide range of conditions. Our papain is a vegetarian and vegan-friendly protease and is non-GMO, as well as Kosher (Kof-K) and Halal (IFANCA) certified.
Enzyme Development Corporation was founded in 1953, with Papain as our first product. It was originally used for chill-proofing/clarifying beer and is still used for this application today.
For more information on papain, please visit Papain.com
Papain activity assays: Milk Clot Unit (MCU) • FCC Botanical Protease Units (FCC-PU) • Tyrosine Assay Method (TU)
EDC is the only producer of Papain in the western hemisphere. At our factory in Scranton, Pennsylvania, we produce standardized Papain powders and liquids, as well as unstandardized concentrates. We also produce a no sulfite added Papain that is OMRI Listed for use in Organic food processing applications. EDC’s Papain products include:
ENZECO® PURIFIED PAPAIN CONCENTRATE
A concentrated papain powder.
ENZECO® PURIFIED PAPAIN RS CONCENTRATE
Papain that is processed without the use of sulfites. The papaya latex in this product has been processed without the addition of sodium metabisulfite. This results in papain with substantially reduced levels of SO2.
This product contains only the botanical enzymes and has no other ingredients. It has earned an OMRI listed® certification, which means that it may be used in certified organic operations or food processing under the USDA National Organic Program.
ENZECO® BROMELAIN
A purified, highly active, soluble proteolytic food grade enzyme that is naturally occurring in the mature pineapple plant stems of the Bromeliaceae family. This is a powdered enzyme that is available at different activity levels and standardized to meet the application and dosage constraints.
Activity assays: Casein Digestion Unit (CDU) • Milk Clot Unit (MCU) • FCC Botanical Protease Units (FCC-PU) • Bromelain Tyrosine Units (BTU)
ENZECO® FUNGAL PROTEASE
A concentrated food-grade fungal proteolytic enzyme, with a high level of endo-protease and lower levels of exo-protease. It is derived from a non-GMO strain of Aspergillus oryzae and is available as a powder or micro-granulate at different activity levels up to 1,000,000 HUT/g.
Activity assays: Hemoglobin Units on Tyrosine basis (HUT)
ENZECO® FUNGAL PROTEASE 400
A unique fungal protease blend from a non-GMO strain of Aspergillus oryzae that contains proteases with an acid pH optimum as well as an alkaline pH optimum. It contains a high level of debittering enzymes and endo-action proteases for flavor enhanced hydrolyzed proteins. This product also contains leucine aminopeptidase (LAP), fungal alkaline protease, fungal neutral protease, and carboxypeptidase.
Activity assays: Hemoglobin Units on Tyrosine basis (HUT)
ENZECO® PROTEASE FNP
A fungal neutral protease preparation derived from a non-GMO strain of Aspergillus oryzae that has optimum activity from pH 5-7 and contains a high level of aminopeptidase activity and acid carboxypeptidase. It also contains a high level of debittering enzymes (Leucine aminopeptidase (LAP) and carboxypeptidase) and endo-action proteases for flavor enhanced hydrolyzed proteins.
Activity assays: Casein Folin A (CFA), Activity can also be reported on Hemoglobin Units on Tyrosine basis (HUT)
ENZECO® FUNGAL ACID PROTEASE
Derived from a non-GMO strain Aspergillus niger, this protease has a pH optimum of 2.5 – 3.5. Its low pH optimum has proven useful in protein hydrolysis as a kosher/halal alternate to pepsin. This product can be used up to a pH of 6.5. At this higher pH, the product can be used for flavor modification and release of free amino nitrogen (FAN).
Activity assays: Sold on Spectrophotometric Acid Protease (SAP). Activity can also be reported on Acid Protease Units (APU), Milk Clot Units (MCU), Hemoglobin Units on Tyrosine (HUT)
ENZECO® DPP (dipeptidyl aminopeptidase)
A protease enzyme preparation derived from a non-GMO strain of Aspergillus oryzae containing high levels of dipeptidyl-aminopeptidase. Primary used in flavor modification, and collagen and protein hydrolysis.
This enzyme must be used with another protease.
Activity assays: DAP or DPP IV
ENZECO® NEUTRAL BACTERIAL PROTEASE 160S
This is a neutral protease derived from Bacillus amyloliquifaciens (formerly B. subtilis) that has optimum activity from pH 5.5-8 and contains no/very low amylase side activity. Hydrolyzes polypeptides to produce lower molecular weight peptides.
Activity assays: Protease Assay (PC)
CARBOHYDRASES
ENZECO® ALPHA GALACTOSIDASE
A food-grade enzyme derived from a non-GMO Strain of Aspergillus niger. It is a di, tri, and oligosaccharide hydrolyzing enzyme, capable of hydrolyzing melibiose, stachyose, and raffinose sugars.
Application: Galactosidase unit (GalU)
ENZECO® FUNGAL ALPHA AMYLASE
A food-grade fungal alpha-amylase enzyme produced from Aspergillus oryzae that breaks down gelatinized starch to dextrins and maltose. Prolonged action results in the formation of large amounts of maltose. It is available in liquid, granulated and powder forms and is available in a range of activities.
Activity assays: Modified SKB method (Sandstedt, Kneen, and Blish, Cereal Chemistry 16:172, 1939)
ENZECO® FUNGAL LACTASE
An acid optimum beta-D-galactoside galactohydrolase (β-galactosidase) derived from a non-GMO strain of Aspergillus oryzae. It hydrolyzes lactose resulting in glucose and galactose sugars. Generally used in dry mixes containing lactose or in lactase type tablets or capsules for nutraceutical applications. It can also be used in the production of galactooligosaccharides (GOS). It is stable at acid pH’s and higher temperatures.
Activity assay: FCC ALU
ENZECO® INVERTASE
A highly active food-grade enzyme derived from the yeast Saccharomyces cerevisiae which converts sucrose to form glucose and fructose.
Activity assay: Sumner Unit
LIPASES
ENZECO® MICROBIAL LIPASE CONCENTRATE
A food-grade enzyme derived from a non-GMO Strain of Aspergillus niger. It is a high activity material. The enzyme will hydrolyze fats and oils most commonly at the 1, 3 positions. This product is heat stable and is stable over a broad and can work up to a pH of 10.
Activity assay: FIPU (Fédération Internationale Pharmaceutique unit)
ENZECO® CANDIDA LIPASE 400
A highly concentrated, unstandardized lipase derived from a non-GMO strain of Candida cylindracea. This lipase hydrolyzes a wide variety of fats and oils and is particularly active at low to moderate temperatures.
Activity assay: FIPU (Fédération Internationale Pharmaceutique unit)
ENZECO® LIPASE RO 20
A unique lipase derived from a non-GMO Strain of Rhizopus oryzae. The enzyme produces significant levels of short chain fatty acids such as C8. This lipase is not as aggressive as lipase from A. niger or pancreatic lipase on long-chain fatty acids.
Activity assay: FIPU (Fédération Internationale Pharmaceutique unit)
CELLULASES/HEMICELLULASES
ENZECO® CELLULASE TR Concentrate
A food-grade fungal cellulase preparation derived from a non-GMO strain of Trichoderma longibrachiatum that is high in cellulase and beta-glucanase activity.
Activity assays: Endo Cellulase Units (ECU)
ENZECO® XYLANASE S CONCENTRATE
Xylanases S Concentrate is our highest xylanase activity product. It is a food-grade enzyme preparation from a non-GMO strain of Trichoderma longibrachiatum (formerly T. reesei), containing xylanase as its principal activity. It also contains beta-glucanase, galactomannanase, and cellulase, and has very little protease activity.
Activity assays: BXU (Birch Xylan Substrate)
ENZECO® MANNANASE CONCENTRATE
A powdered food-grade enzyme preparation derived from a non-GMO strain of Aspergillus niger. It contains significant amounts of hemicellulase, mannanase, guarase, xylanase and also has some cellulase and β-glucanase.
Activity assays: ACH
OTHER PROPRIETARY BLENDS
ENZOPHARM® PLUS
Proprietary blend of enzymes standardized to contain high amylase and protease activity as well as cellulase, hemicellulase, lipase, and lactase activity.